Animal models yield irreproducible results: reproduction is the most divergent function between species.<\/li>\n<\/ul>\n[\/vc_column_text][image_with_animation image_url=”5935″ image_size=”full” animation_type=”entrance” animation=”None” animation_movement_type=”transform_y” hover_animation=”none” alignment=”” border_radius=”none” box_shadow=”none” image_loading=”default” max_width=”100%” max_width_mobile=”default”][image_with_animation image_url=”5936″ image_size=”full” animation_type=”entrance” animation=”None” animation_movement_type=”transform_y” hover_animation=”none” alignment=”” border_radius=”none” box_shadow=”none” image_loading=”default” max_width=”100%” max_width_mobile=”default”][vc_column_text]\nb) ORHUMAN Project<\/h3>\n
<\/p>\n
ORHUMAN: HU<\/strong>man M<\/strong>ale RE<\/strong>productive OR<\/strong>ganoids<\/p>\nThe solution: organoids of seminiferous tubules, epididymis, and deferens.<\/p>\n
\n- Organoids are three-dimensional structures derived from adult or pluripotent stem cells.<\/li>\n
- Fundamental characteristic: the capacity for self-organization<\/strong> of cultured cells, reminiscent of a developmental program.<\/li>\n
- Obtaining a miniaturized pseudo-organ (200\u03bcm to 1000\u03bcm) hosting all cell types of the organ in question and exhibiting a function similar to the original tissue<\/li>\n<\/ul>\n[\/vc_column_text][image_with_animation image_url=”5937″ image_size=”full” animation_type=”entrance” animation=”None” animation_movement_type=”transform_y” hover_animation=”none” alignment=”” border_radius=”none” box_shadow=”none” image_loading=”default” max_width=”100%” max_width_mobile=”default”][image_with_animation image_url=”5939″ image_size=”full” animation_type=”entrance” animation=”None” animation_movement_type=”transform_y” hover_animation=”none” alignment=”” border_radius=”none” box_shadow=”none” image_loading=”default” max_width=”100%” max_width_mobile=”default”][vc_column_text]The solution: human organoids of seminiferous tubules, epididymis, and deferens.<\/p>\n
\n- Seminiferous tubule organoids: pluripotent cells; the culture method to differentiate iPSCs (or ECs) into Sertoli cells is known (addition of BMP4, FGF9, Activin A to culture media)<\/li>\n
- Epididymis and deferens organoids: adult stem cells currently being characterized (double labeling* – intracellular and surface)<\/li>\n
- A major innovation used: a novel culture process (non-public due to patent)<\/li>\n<\/ul>\n
Cells will be obtained from brain-dead patients (PMO) -> the protocol is approved and registered with the ABM under No. PFS19-010.[\/vc_column_text][image_with_animation image_url=”5938″ image_size=”full” animation_type=”entrance” animation=”None” animation_movement_type=”transform_y” hover_animation=”none” alignment=”” border_radius=”none” box_shadow=”none” image_loading=”default” max_width=”100%” max_width_mobile=”default”][vc_column_text]Human male tract organoids (Dr. G. Perez, R. Soudy. DEFE)<\/strong>[\/vc_column_text][image_with_animation image_url=”6060″ image_size=”full” animation_type=”entrance” animation=”None” animation_movement_type=”transform_y” hover_animation=”none” alignment=”” border_radius=”none” box_shadow=”none” image_loading=”default” max_width=”100%” max_width_mobile=”default”][vc_column_text]\n\n- Funding: <\/strong>Occitanie Region and Urosph\u00e8re SAS company (36-month renewable funding)<\/li>\n
- Partnership: <\/strong>Urosphere SAS and Inserm U1220 (hosts the \u201cOrganoids\u201d platform labeled GIS IBiSA). A collaboration in 2016 between Urosphere and U1220 led to the development of the first human bladder organoid cultures. <\/li>\n
- ORHUMAN Duration<\/strong>: Minimum 5 years, starting February 2020.<\/li>\n
- Personnel: <\/strong>\n
\n- One postdoctoral researcher is recruited full-time for the project<\/li>\n
- One research engineer from Urosphere is assigned to the project<\/li>\n
- One to two doctoral students will be trained through the project<\/li>\n
- Interns will be hosted throughout the project period<\/li>\n
- A training module in doctoral school (ED BSB) may be offered.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n[\/vc_column_text][\/tab][\/tabbed_section][\/vc_column][\/vc_row]\n","protected":false},"excerpt":{"rendered":"
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